Chapter 1531 Gretel, you finally look like an evocation mage!
Chapter 1531 Gretel, you finally look like an evocation mage!
Chapter 1531 Gretel, you finally look like an evocation mage!
(ScarletC million rewards plus more)
One hundred microinjections a day.
Ten days, a thousand times in total.
Gretel did the experiment with a scowl.After finishing the experiment and analyzing the reasons for the failure of the experiment, I became even more frowning:
"The needle was inserted too hard, the angle was wrong, and the fertilized egg was pierced... This data must be excluded..."
"Extracted too much cytoplasm? Do you want to control the amount of extraction next time?"
"Or, the nucleus was accidentally extracted? The part sent into the fertilized egg contained chromosomes, and the result was a triploid?"
"Or, the fertilized egg was poked a hole, which hurt it too much? Do you want to give it a healing technique to let the cell membrane heal naturally?"
"Or, should we beat the unfertilized eggs into a paste, put the fertilized eggs in it, and let it absorb nutrients naturally?"
"Ah... so difficult... How can I make the fertilized egg feel that the things sent in are for it to ingest..."
Gretel clasped his head in his hands and slammed his head on the experiment table, humming.From behind, Cerilla walked over lightly, reached out and rubbed his shoulders:
"Okay, if we can't figure it out, let's change our thinking. Or it's okay to slow down, don't be so anxious, you can see that your eyes are blurred... It doesn't matter if you don't make a contract, I always believe in you!"
Gretel took a long breath, straightened up, wanted to lean back, and stopped at the last moment before reaching Cirella.He leaned forward again, burying his forehead between his hands:
"It's not for this...Cerilla, it's not for this. My instinct is that this road should work, but I haven't found a way yet... There must be a way, there must be a way..."
In the previous life, even the genetic material was taken out of the animal body cells, and then stuffed into the egg cells to promote the development of the egg cells, what else could not be done?It must be that he didn't find the right way...
But where is the method?He is not an expert in cloning, he is not even an expert in cell research, and he has never even touched the profession of biomedicine!
"If you can't think of a way, just do it indiscriminately." Regarding this, Cerilla was very calm, even a little indifferent:
"Didn't you say that, Gretel? The so-called research is to follow the idea if you have an idea, and to mess around if you don't have an idea~~~ Especially when making materials, you just have all the materials at hand, this, that, and so on. Pour inside..."
"Stop, stop, stop --" Gretel interrupted with a headache and raised his hand:
"When did I say such a thing?"
"That's when Mr. Norwood was studying that... that... that rubber!" Cerilla said confidently:
"At that time, he had no idea. That's what you said. There are so many materials in the mage's tower, just pour them all into the rubber!"
"But it's not good to study fertilized eggs." Gretel didn't know whether to laugh or cry:
"For a delicate thing like a fertilized egg, it will die if the temperature is a little higher, and it will die if it is a little lower; if the culture solution is a little thicker, it will die, and if it is a little lighter, it will die..."
It's like growing bacteria in a laboratory.Obviously, it grows particularly vigorously in the outside world, and a large colony of bacteria can grow on a salted fish. In the laboratory, the temperature is 0.1 degrees higher today, ah, I am dead...
The experimenters who were in charge of raising the bacteria almost grabbed the ground with their heads and burst into tears.
"If you die, you will die!" At this time, Cerilla fully demonstrated the arrogance of the dragon race as a superior race, or in other words, the willfulness of the eldest lady as the little princess of the silver dragon.He raised his chin and waved his hand vigorously:
"Anyway, these devil frog eggs are going to die! Anyway, they hatched normally, and a large part of them will not hatch, and a large part of them will not grow to adulthood! Gretel, you can do whatever you want!
Adding acid, alkali and salt, heating and freezing, gravity and magnetic current...all of them!I think there is always a way to do it! "
"Hmm..." Gretel held his chin and really thought about it seriously.Adding acid, alkali and salt may not work, as fertilized eggs are corroded, and problems will occur in minutes;
You can try heating and freezing, but you can only try it slightly, and you can't denature the protein, crystallize the water in the cell, or puncture the organelle;
Gravity, magnetism, electric current, and ultraviolet rays, all kinds of rays, in fact, you can try them all!
It's done!
Gretel jumped up and began to experiment with a broken can.Put it into the water bath, increase the temperature by 0.1 degrees, then increase it by 0.1 degrees, then increase it by 0.1 degrees... increase it little by little;
Then put it into the freezing magic circle, the temperature will drop by 0.1 degrees, then 0.1 degrees, and then 0.1 degrees...
The gravitational field is more troublesome, so I simply put it directly into the centrifuge, using centrifugal force instead of gravity, and turning round and round;
The coil is set up, the magnetic field is pulled up, the pulse current is passed through, and the ultraviolet rays are illuminated; there is another laboratory next to it, and all kinds of radiation, alpha rays, beta rays, gamma rays, are illuminated one by one!
After such a series of experiments, I finally saw various changes in the fertilized eggs of the magic frog.The vast majority are hatching failures, or hatching deformities, or the fertilized eggs are halfway through;
There is also radiation, and the chromosomes inside are completely disintegrated and cannot be further divided, such as the result of strong ultraviolet radiation;
However, the result of pulse current stimulation really gave Grete a little surprise:
"Cerilla, look!" Gretel lay prone in front of the microscope, his eyes sparkling:
"The fertilized eggs seem to be developing better!"
"Where? Where is where?"
Cerilla quickly pushed aside, and lay down in front of the microscope, trying to observe.However, she didn't follow the whole process like Gretel, and unlike Gretel, she carefully observed every fertilized egg with a microscope and accumulated sufficient experience.
She looked left, right, up, and down, but she didn't see anything. She could only look back at Gretel in a daze:
"...Where did you see it?"
"That's right, it looks stronger." Gretel didn't know how to describe it to her for a while, so he could only make a few gestures feebly:
"In short, you see, according to the previous records, it should have developed to the blastocyst stage in an hour. But now, it has reached the blastocyst stage, and the speed is so fast! So, give it a little electrical stimulation, and it will Sure enough, it can grow better!"
"That's great!" Although Cerilla couldn't even understand the difference between a fertilized egg and a blastocyst, seeing Gretel happy, her face lit up brightly:
"Then power it up! Power it up hard! Power it up a few times, use various methods, try to power it up as much as possible!"
Gretel was also greatly encouraged.Since giving electrical stimulation can make the fertilized eggs develop better, we should give electrical stimulation in different ways.
——It is best to be able to sum up an effective method, even magic, so that magicians can provide services for female dragons that need to be conceived.
If the female dragon can learn to look inside her own ovaries and fallopian tubes and operate them for herself, that would be very good of course...
With such expectations, Gretel threw himself into the research with great vigor.Then, he encountered a string of failures:
"The fertilized egg is finished, and there is a big hole in the cell membrane... Is the current too strong?"
"What is the current intensity...Wait, I remember something called transmembrane voltage, I have to think about what it is...Theoretically, it should be just above the transmembrane voltage, just enough to wrap the cell membrane Piercing!"
"The fertilized egg is finished again... This time it doesn't seem to be a problem with the current, could it be a problem with the pulse frequency? Well, adjust the pulse frequency again..."
"It's over again... I know, fertilized eggs and unfertilized eggs must be close to each other, and there is no distance at all! If there is a short distance, ten times the current must be used to make up, and the fertilized eggs will be burned !"
"Uh, the flow of cytoplasm is reversed... It's actually from fertilized eggs to non-fertilized eggs, this is not what I want! I'm not doing cloning... I know, the direction of the pulse current is reversed!"
"What's wrong this time... Wait, the direction of the pulse current is crooked! It's crooked! If the direction is crooked, it will cause unnecessary big holes... But how to determine its direction... The micro-ranging magic circle How to set it up?"
"It still doesn't work, too many egg cells are dead, and both ends are perforating... I know, the transmembrane voltage of the two poles of the cell membrane and the contact area exceeds the perforation threshold, this is not acceptable!
The damage can only be minimized by letting the small area of contact between fertilized eggs and unfertilized eggs exceed the perforation threshold!But what to do..."
Gretel tossed and tossed the current to death.After tossing and tossing for half a month, Cirella was unwilling to approach him——
Even when he was walking in the mage's tower, there were electric charges floating around him unconsciously, crackling, and sparks would be shot out when he touched it.
On the contrary, Archmage Philby greatly appreciated Gretel.That's right, as a magician of the evocation department, as a magician of the Horn of Thunder, everyone has such a time!
It's been a long time, and this is the first time she's seen that the junior is devoted to the research of the direction of electric current!
Little Gretel, you finally look like a Thunderhorn mage!
"Little Gretel, how are you doing?" Seizing a chance when no one was around, Archmage Philby stopped Gretel and asked with a smile.Gretel scratched his hair in annoyance:
"Senior Sister, come and take a look... I can't break through the last level..."
In view of the fact that the junior brother finally focused on the study of lightning spells, Archmage Philbi was in a good mood, and patted his chest boldly:
"Don't worry, I'll leave it to the senior sister. If you encounter any problems, the senior sister will help you solve it!"
Then, Your Excellency the Archmage, he plunged into things like current, voltage, pulse frequency, electric field strength, waveform...
"Don't worry, I will definitely be able to research it! Give me another three days!"
"Another three days!"
"Another three days!"
Gretel: ...Sister, come on! ! !
This chapter refers to "Effects of different electrofusion conditions on the fusion and early development of mouse cumulus cell nuclear transfer recombinant embryos", "Production of transgenic fish by electric pulse method", "Simulation and experimental research on improving the efficiency of cell electrofusion with nano-microsecond compound pulses ", "Electric Pulse-Assisted Activation of Human Oocytes", "Research on High-throughput Cell Electrofusion Method Based on Microelectrode Arrays" and other papers... exhausted...
(End of this chapter)
Phi-Fic